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plasmids 21807 21809  (Addgene inc)


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    Addgene inc plasmids 21807 21809
    Plasmids 21807 21809, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmids+21807+21809/eIF2a+3+(Plasmid+%2321809)/10__1074_slash_jbc__m112__379883-57-50-49
    Average 93 stars, based on 6 article reviews
    plasmids 21807 21809 - by Bioz Stars, 2026-09
    93/100 stars

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    other:

    Article Title: A Subunit of Eukaryotic Translation Initiation Factor 2?-Phosphatase (CreP/PPP1R15B) Regulates Membrane Traffic
    Article Snippet: The following small interfering RNAs and control siRNA were from Qiagen: PPP1R15B 1, 5′-AAGGGAUGGAUGCAGGUUCCA-3′ ( 6 ); PPP1R15B 2, 5′-CCGAAUAAGUGUAGUUGAUUA-3′; eIF2α-5, 5′-GGCUGUAAAUCCUAGACUUTT-3′; eIF2α-7, 5′-GGCGUAUCCGUUCUAUCAATT-3′; GCN2, 5′-CAAGGUUAAGUCUUUCGAGAA-3′.

    Article Title: A Subunit of Eukaryotic Translation Initiation Factor 2?-Phosphatase (CreP/PPP1R15B) Regulates Membrane Traffic
    Article Snippet: Antibodies against p-eIF2α and eIF2α were from Abcam (immunofluorescence) and Cell Signaling Technology (Western blot).

    Polymerase Chain Reaction:

    Article Title: A Subunit of Eukaryotic Translation Initiation Factor 2?-Phosphatase (CreP/PPP1R15B) Regulates Membrane Traffic
    Article Snippet: Plasmid encoding human CReP was purchased from imaGenes, and CReP cDNA was subcloned into p3xFLAG-CMV-10 (Sigma), EGFP-C1, or dsRed-C1 (Clontech). .. EGFP-tagged, truncated versions of CReP were made by PCR amplification of the respective fragments and subcloning into pEGFP-C1. pEGFP-eIF2α, pEGFP-eIF2αS51A, and pEGFP-eIF2αS51D were generated by excision of the corresponding cDNAs from pCDNA3-CD2-eIF2αwt or the corresponding plasmids carrying the mutant versions of eIF2α, obtained from Addgene (plasmids 21807–21809; Dr. David Ron), and cloning into pEGFP-C1 (Clontech). pEGFP-eIF2αS51D-NTD and pEGFP-eIF2α-CTD encode the EGFP-tagged N-terminal domain of eIF2αS51D and the EGFP-tagged C terminus of eIF2α, respectively; these plasmids were made by PCR amplification of the N- or C-terminal halves of EGFP-eIF2αS51D, TA cloning into pGEM-T (Promega), and subcloning into pEGFP-C1. ..

    Article Title: A Subunit of Eukaryotic Translation Initiation Factor 2α-Phosphatase (CreP/PPP1R15B) Regulates Membrane Traffic
    Article Snippet: Plasmid encoding human CReP was purchased from imaGenes, and CReP cDNA was subcloned into p3xFLAG-CMV-10 (Sigma), EGFP-C1, or dsRed-C1 (Clontech). .. EGFP-tagged, truncated versions of CReP were made by PCR amplification of the respective fragments and subcloning into pEGFP-C1. pEGFP-eIF2 , pEGFP-eIF2 S51A, and pEGFP-eIF2 S51D were generated by excision of the corresponding cDNAs from pCDNA3-CD2-eIF2 wt or the corresponding plasmids carrying the mutant versions of eIF2 , obtained from Addgene (plasmids 21807–21809; Dr. David Ron), and cloning into pEGFP-C1 (Clontech). pEGFPeIF2 S51D-NTD and pEGFP-eIF2 -CTD encode the EGFPtagged N-terminal domain of eIF2 S51D and the EGFP-tagged C terminus of eIF2 , respectively; these plasmids weremade by PCR amplification of the N- or C-terminal halves of EGFPeIF2 S51D, TA cloning into pGEM-T (Promega), and subcloning into pEGFP-C1. ..

    Amplification:

    Article Title: A Subunit of Eukaryotic Translation Initiation Factor 2?-Phosphatase (CreP/PPP1R15B) Regulates Membrane Traffic
    Article Snippet: Plasmid encoding human CReP was purchased from imaGenes, and CReP cDNA was subcloned into p3xFLAG-CMV-10 (Sigma), EGFP-C1, or dsRed-C1 (Clontech). .. EGFP-tagged, truncated versions of CReP were made by PCR amplification of the respective fragments and subcloning into pEGFP-C1. pEGFP-eIF2α, pEGFP-eIF2αS51A, and pEGFP-eIF2αS51D were generated by excision of the corresponding cDNAs from pCDNA3-CD2-eIF2αwt or the corresponding plasmids carrying the mutant versions of eIF2α, obtained from Addgene (plasmids 21807–21809; Dr. David Ron), and cloning into pEGFP-C1 (Clontech). pEGFP-eIF2αS51D-NTD and pEGFP-eIF2α-CTD encode the EGFP-tagged N-terminal domain of eIF2αS51D and the EGFP-tagged C terminus of eIF2α, respectively; these plasmids were made by PCR amplification of the N- or C-terminal halves of EGFP-eIF2αS51D, TA cloning into pGEM-T (Promega), and subcloning into pEGFP-C1. ..

    Article Title: A Subunit of Eukaryotic Translation Initiation Factor 2α-Phosphatase (CreP/PPP1R15B) Regulates Membrane Traffic
    Article Snippet: Plasmid encoding human CReP was purchased from imaGenes, and CReP cDNA was subcloned into p3xFLAG-CMV-10 (Sigma), EGFP-C1, or dsRed-C1 (Clontech). .. EGFP-tagged, truncated versions of CReP were made by PCR amplification of the respective fragments and subcloning into pEGFP-C1. pEGFP-eIF2 , pEGFP-eIF2 S51A, and pEGFP-eIF2 S51D were generated by excision of the corresponding cDNAs from pCDNA3-CD2-eIF2 wt or the corresponding plasmids carrying the mutant versions of eIF2 , obtained from Addgene (plasmids 21807–21809; Dr. David Ron), and cloning into pEGFP-C1 (Clontech). pEGFPeIF2 S51D-NTD and pEGFP-eIF2 -CTD encode the EGFPtagged N-terminal domain of eIF2 S51D and the EGFP-tagged C terminus of eIF2 , respectively; these plasmids weremade by PCR amplification of the N- or C-terminal halves of EGFPeIF2 S51D, TA cloning into pGEM-T (Promega), and subcloning into pEGFP-C1. ..

    Subcloning:

    Article Title: A Subunit of Eukaryotic Translation Initiation Factor 2?-Phosphatase (CreP/PPP1R15B) Regulates Membrane Traffic
    Article Snippet: Plasmid encoding human CReP was purchased from imaGenes, and CReP cDNA was subcloned into p3xFLAG-CMV-10 (Sigma), EGFP-C1, or dsRed-C1 (Clontech). .. EGFP-tagged, truncated versions of CReP were made by PCR amplification of the respective fragments and subcloning into pEGFP-C1. pEGFP-eIF2α, pEGFP-eIF2αS51A, and pEGFP-eIF2αS51D were generated by excision of the corresponding cDNAs from pCDNA3-CD2-eIF2αwt or the corresponding plasmids carrying the mutant versions of eIF2α, obtained from Addgene (plasmids 21807–21809; Dr. David Ron), and cloning into pEGFP-C1 (Clontech). pEGFP-eIF2αS51D-NTD and pEGFP-eIF2α-CTD encode the EGFP-tagged N-terminal domain of eIF2αS51D and the EGFP-tagged C terminus of eIF2α, respectively; these plasmids were made by PCR amplification of the N- or C-terminal halves of EGFP-eIF2αS51D, TA cloning into pGEM-T (Promega), and subcloning into pEGFP-C1. ..

    Article Title: A Subunit of Eukaryotic Translation Initiation Factor 2α-Phosphatase (CreP/PPP1R15B) Regulates Membrane Traffic
    Article Snippet: Plasmid encoding human CReP was purchased from imaGenes, and CReP cDNA was subcloned into p3xFLAG-CMV-10 (Sigma), EGFP-C1, or dsRed-C1 (Clontech). .. EGFP-tagged, truncated versions of CReP were made by PCR amplification of the respective fragments and subcloning into pEGFP-C1. pEGFP-eIF2 , pEGFP-eIF2 S51A, and pEGFP-eIF2 S51D were generated by excision of the corresponding cDNAs from pCDNA3-CD2-eIF2 wt or the corresponding plasmids carrying the mutant versions of eIF2 , obtained from Addgene (plasmids 21807–21809; Dr. David Ron), and cloning into pEGFP-C1 (Clontech). pEGFPeIF2 S51D-NTD and pEGFP-eIF2 -CTD encode the EGFPtagged N-terminal domain of eIF2 S51D and the EGFP-tagged C terminus of eIF2 , respectively; these plasmids weremade by PCR amplification of the N- or C-terminal halves of EGFPeIF2 S51D, TA cloning into pGEM-T (Promega), and subcloning into pEGFP-C1. ..

    Generated:

    Article Title: A Subunit of Eukaryotic Translation Initiation Factor 2?-Phosphatase (CreP/PPP1R15B) Regulates Membrane Traffic
    Article Snippet: Plasmid encoding human CReP was purchased from imaGenes, and CReP cDNA was subcloned into p3xFLAG-CMV-10 (Sigma), EGFP-C1, or dsRed-C1 (Clontech). .. EGFP-tagged, truncated versions of CReP were made by PCR amplification of the respective fragments and subcloning into pEGFP-C1. pEGFP-eIF2α, pEGFP-eIF2αS51A, and pEGFP-eIF2αS51D were generated by excision of the corresponding cDNAs from pCDNA3-CD2-eIF2αwt or the corresponding plasmids carrying the mutant versions of eIF2α, obtained from Addgene (plasmids 21807–21809; Dr. David Ron), and cloning into pEGFP-C1 (Clontech). pEGFP-eIF2αS51D-NTD and pEGFP-eIF2α-CTD encode the EGFP-tagged N-terminal domain of eIF2αS51D and the EGFP-tagged C terminus of eIF2α, respectively; these plasmids were made by PCR amplification of the N- or C-terminal halves of EGFP-eIF2αS51D, TA cloning into pGEM-T (Promega), and subcloning into pEGFP-C1. ..

    Article Title: A Subunit of Eukaryotic Translation Initiation Factor 2α-Phosphatase (CreP/PPP1R15B) Regulates Membrane Traffic
    Article Snippet: Plasmid encoding human CReP was purchased from imaGenes, and CReP cDNA was subcloned into p3xFLAG-CMV-10 (Sigma), EGFP-C1, or dsRed-C1 (Clontech). .. EGFP-tagged, truncated versions of CReP were made by PCR amplification of the respective fragments and subcloning into pEGFP-C1. pEGFP-eIF2 , pEGFP-eIF2 S51A, and pEGFP-eIF2 S51D were generated by excision of the corresponding cDNAs from pCDNA3-CD2-eIF2 wt or the corresponding plasmids carrying the mutant versions of eIF2 , obtained from Addgene (plasmids 21807–21809; Dr. David Ron), and cloning into pEGFP-C1 (Clontech). pEGFPeIF2 S51D-NTD and pEGFP-eIF2 -CTD encode the EGFPtagged N-terminal domain of eIF2 S51D and the EGFP-tagged C terminus of eIF2 , respectively; these plasmids weremade by PCR amplification of the N- or C-terminal halves of EGFPeIF2 S51D, TA cloning into pGEM-T (Promega), and subcloning into pEGFP-C1. ..

    Mutagenesis:

    Article Title: A Subunit of Eukaryotic Translation Initiation Factor 2?-Phosphatase (CreP/PPP1R15B) Regulates Membrane Traffic
    Article Snippet: Plasmid encoding human CReP was purchased from imaGenes, and CReP cDNA was subcloned into p3xFLAG-CMV-10 (Sigma), EGFP-C1, or dsRed-C1 (Clontech). .. EGFP-tagged, truncated versions of CReP were made by PCR amplification of the respective fragments and subcloning into pEGFP-C1. pEGFP-eIF2α, pEGFP-eIF2αS51A, and pEGFP-eIF2αS51D were generated by excision of the corresponding cDNAs from pCDNA3-CD2-eIF2αwt or the corresponding plasmids carrying the mutant versions of eIF2α, obtained from Addgene (plasmids 21807–21809; Dr. David Ron), and cloning into pEGFP-C1 (Clontech). pEGFP-eIF2αS51D-NTD and pEGFP-eIF2α-CTD encode the EGFP-tagged N-terminal domain of eIF2αS51D and the EGFP-tagged C terminus of eIF2α, respectively; these plasmids were made by PCR amplification of the N- or C-terminal halves of EGFP-eIF2αS51D, TA cloning into pGEM-T (Promega), and subcloning into pEGFP-C1. ..

    Article Title: A Subunit of Eukaryotic Translation Initiation Factor 2α-Phosphatase (CreP/PPP1R15B) Regulates Membrane Traffic
    Article Snippet: Plasmid encoding human CReP was purchased from imaGenes, and CReP cDNA was subcloned into p3xFLAG-CMV-10 (Sigma), EGFP-C1, or dsRed-C1 (Clontech). .. EGFP-tagged, truncated versions of CReP were made by PCR amplification of the respective fragments and subcloning into pEGFP-C1. pEGFP-eIF2 , pEGFP-eIF2 S51A, and pEGFP-eIF2 S51D were generated by excision of the corresponding cDNAs from pCDNA3-CD2-eIF2 wt or the corresponding plasmids carrying the mutant versions of eIF2 , obtained from Addgene (plasmids 21807–21809; Dr. David Ron), and cloning into pEGFP-C1 (Clontech). pEGFPeIF2 S51D-NTD and pEGFP-eIF2 -CTD encode the EGFPtagged N-terminal domain of eIF2 S51D and the EGFP-tagged C terminus of eIF2 , respectively; these plasmids weremade by PCR amplification of the N- or C-terminal halves of EGFPeIF2 S51D, TA cloning into pGEM-T (Promega), and subcloning into pEGFP-C1. ..

    Clone Assay:

    Article Title: A Subunit of Eukaryotic Translation Initiation Factor 2?-Phosphatase (CreP/PPP1R15B) Regulates Membrane Traffic
    Article Snippet: Plasmid encoding human CReP was purchased from imaGenes, and CReP cDNA was subcloned into p3xFLAG-CMV-10 (Sigma), EGFP-C1, or dsRed-C1 (Clontech). .. EGFP-tagged, truncated versions of CReP were made by PCR amplification of the respective fragments and subcloning into pEGFP-C1. pEGFP-eIF2α, pEGFP-eIF2αS51A, and pEGFP-eIF2αS51D were generated by excision of the corresponding cDNAs from pCDNA3-CD2-eIF2αwt or the corresponding plasmids carrying the mutant versions of eIF2α, obtained from Addgene (plasmids 21807–21809; Dr. David Ron), and cloning into pEGFP-C1 (Clontech). pEGFP-eIF2αS51D-NTD and pEGFP-eIF2α-CTD encode the EGFP-tagged N-terminal domain of eIF2αS51D and the EGFP-tagged C terminus of eIF2α, respectively; these plasmids were made by PCR amplification of the N- or C-terminal halves of EGFP-eIF2αS51D, TA cloning into pGEM-T (Promega), and subcloning into pEGFP-C1. ..

    TA Cloning:

    Article Title: A Subunit of Eukaryotic Translation Initiation Factor 2?-Phosphatase (CreP/PPP1R15B) Regulates Membrane Traffic
    Article Snippet: Plasmid encoding human CReP was purchased from imaGenes, and CReP cDNA was subcloned into p3xFLAG-CMV-10 (Sigma), EGFP-C1, or dsRed-C1 (Clontech). .. EGFP-tagged, truncated versions of CReP were made by PCR amplification of the respective fragments and subcloning into pEGFP-C1. pEGFP-eIF2α, pEGFP-eIF2αS51A, and pEGFP-eIF2αS51D were generated by excision of the corresponding cDNAs from pCDNA3-CD2-eIF2αwt or the corresponding plasmids carrying the mutant versions of eIF2α, obtained from Addgene (plasmids 21807–21809; Dr. David Ron), and cloning into pEGFP-C1 (Clontech). pEGFP-eIF2αS51D-NTD and pEGFP-eIF2α-CTD encode the EGFP-tagged N-terminal domain of eIF2αS51D and the EGFP-tagged C terminus of eIF2α, respectively; these plasmids were made by PCR amplification of the N- or C-terminal halves of EGFP-eIF2αS51D, TA cloning into pGEM-T (Promega), and subcloning into pEGFP-C1. ..

    Article Title: A Subunit of Eukaryotic Translation Initiation Factor 2α-Phosphatase (CreP/PPP1R15B) Regulates Membrane Traffic
    Article Snippet: Plasmid encoding human CReP was purchased from imaGenes, and CReP cDNA was subcloned into p3xFLAG-CMV-10 (Sigma), EGFP-C1, or dsRed-C1 (Clontech). .. EGFP-tagged, truncated versions of CReP were made by PCR amplification of the respective fragments and subcloning into pEGFP-C1. pEGFP-eIF2 , pEGFP-eIF2 S51A, and pEGFP-eIF2 S51D were generated by excision of the corresponding cDNAs from pCDNA3-CD2-eIF2 wt or the corresponding plasmids carrying the mutant versions of eIF2 , obtained from Addgene (plasmids 21807–21809; Dr. David Ron), and cloning into pEGFP-C1 (Clontech). pEGFPeIF2 S51D-NTD and pEGFP-eIF2 -CTD encode the EGFPtagged N-terminal domain of eIF2 S51D and the EGFP-tagged C terminus of eIF2 , respectively; these plasmids weremade by PCR amplification of the N- or C-terminal halves of EGFPeIF2 S51D, TA cloning into pGEM-T (Promega), and subcloning into pEGFP-C1. ..

    Cloning:

    Article Title: A Subunit of Eukaryotic Translation Initiation Factor 2α-Phosphatase (CreP/PPP1R15B) Regulates Membrane Traffic
    Article Snippet: Plasmid encoding human CReP was purchased from imaGenes, and CReP cDNA was subcloned into p3xFLAG-CMV-10 (Sigma), EGFP-C1, or dsRed-C1 (Clontech). .. EGFP-tagged, truncated versions of CReP were made by PCR amplification of the respective fragments and subcloning into pEGFP-C1. pEGFP-eIF2 , pEGFP-eIF2 S51A, and pEGFP-eIF2 S51D were generated by excision of the corresponding cDNAs from pCDNA3-CD2-eIF2 wt or the corresponding plasmids carrying the mutant versions of eIF2 , obtained from Addgene (plasmids 21807–21809; Dr. David Ron), and cloning into pEGFP-C1 (Clontech). pEGFPeIF2 S51D-NTD and pEGFP-eIF2 -CTD encode the EGFPtagged N-terminal domain of eIF2 S51D and the EGFP-tagged C terminus of eIF2 , respectively; these plasmids weremade by PCR amplification of the N- or C-terminal halves of EGFPeIF2 S51D, TA cloning into pGEM-T (Promega), and subcloning into pEGFP-C1. ..



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    plasmids 21807 21809  (Addgene inc)
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    Addgene inc plasmids 21807 21809
    Plasmids 21807 21809, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmids+21807+21809/eIF2a+3+(Plasmid+%2321809)/10__1074_slash_jbc__m112__379883-57-50-49
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    		  Buy from Supplier

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